Together, our data suggest that vitamin E can be used to increase killing by bactericidal antibiotics through interference with lipocalin binding. These results were replicated in vivo using the Galleria mellonella larva infection model whereby vitamin E treatment, in combination with norfloxacin, significantly increased larva survival upon infection in a BcnA-dependent manner. Expression of BcnA was required for the adjuvant effect of vitamin E. Our results demonstrate that in vitro, both vitamin E forms bind strongly to BcnA and contribute to reduce the MICs of norfloxacin (a fluoroquinolone) and ceftazidime (a β-lactam), both of them used as model molecules representing two different chemical classes of antibiotics. In this study, we investigated water-soluble and liposoluble forms of vitamin E as inhibitors of antibiotic binding by BcnA. BcnA captures a range of antibiotics outside bacterial cells, providing a global extracellular mechanism of antimicrobial resistance. cenocepacia produces the extracellular bacterial lipocalin protein BcnA upon exposure to sublethal concentrations of bactericidal antibiotics. All rights reserved.Burkholderia cenocepacia is an opportunistic Gram-negative bacterium that causes serious respiratory infections in patients with cystic fibrosis. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This supports the hypothesis that vitamin D has a clinically relevant effect on the immune system.Īustralian New Zealand Clinical Trials Registry: ACTRN12613000743763.Īntibiotic use randomized controlled trial urinary tract infection vitamin D supplementation. Vitamin D may reduce the number of antibiotic prescriptions, particularly in people with low vitamin D status. There was stronger evidence of benefit in people predicted to have insufficient vitamin D at baseline (prescription episodes IRR, 0.93 95% CI. 93-1.00), and repeat prescription episodes (IRR, 0.96 95% CI. ![]() Vitamin D supplementation slightly reduced the number of prescription episodes (IRR, 0.98 95% confidence interval. We estimated incidence rate ratios (IRRs) using negative binomial regression, and odds ratios using logistic regression. For this analysis, the primary outcome was the number of antibiotic prescription episodes secondary outcomes were total number of prescriptions, repeat prescription episodes, and antibiotics for urinary tract infection. The D-Health Trial is a randomized, double-blind, placebo-controlled trial in which 21 315 Australians aged 60-84 years were randomized to 60 000 IU of supplementary vitamin D3 or placebo monthly for 5 years. We analyzed data from the D-Health Trial, using prescription of antibiotics as a surrogate for infection. Vitamin D supplementation may reduce the risk or severity of infection, but this has been investigated in few large population-based trials.
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